Biology researchers at Indiana University have developed an integrated system that merges high-resolution optical coherence microscopy (HR-OCM) with confocal scanning dual-channel fluorescence microscopy (DC-SCFM) to enable a 3D visual assessment of cellular activities involved in pupil development and pathological conditions. . Still in its experimental stages, this dual-modality 3D system simultaneously co-registers reflectance and fluorescence signals, allowing it to accurately track structural and functional changes in living specimens over time. Indiana University researchers hope to use their system to enable further investigations of biological processes in small animal models.
A BitFlow Axion CameraLink frame grabber is an essential component of the hybrid system. It acquires the output signal from a spectrometer equipped with a Teledyne e2v high-speed line scan camera operating at the frequency of 250 kHz. Lateral resolution of 2 μm and axial resolution of 2.4 μm are captured in tissue over a field of view of 1.1 mm × 1.1 mm. In addition to the frame grabber trigger signals, simultaneous recording of HR-OCM and DC-SCFM data was performed using custom software developed in LabVIEW 2017. Data was performed in LabVIEW and MATLAB.
As the data generated by faster, higher-resolution CameraLink cameras continues to grow exponentially, Axion’s PCIe Gen 2 interface, with its StreamSync DMA optimized for modern computers, is needed to maximize their all-round performance. . Features such as easier switching between different socket formats, a powerful acquisition engine, and a more flexible I/O and timing generator are all readily available in a dedicated CL Base oriented frame grabber Low cost.
During development, researchers applied different strategies to enable simultaneous recording of information, as well as to overcome the focal plane mismatch between the two imaging modalities. The performance of the system was evaluated in imaging fluorescence microspheres embedded in a multilayer tape and a silicone phantom.
The combined system is synergistic in generating structural and functional information from samples; DC-SCFM allows discrimination between different fluorophores, while HR-OCM allows 3D localization of features within tissue samples and enables localization in depth.